c1005 staining solution (Beyotime)
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c1005 staining solution
C1005 Staining Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 2090 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+stain/DAPI+Staining+Solution/pm42880593-220-13-19
Average 99 stars, based on 2090 article reviews
C1005 Staining Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 2090 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+stain/DAPI+Staining+Solution/pm42880593-220-13-19
Average 99 stars, based on 2090 article reviews
c1005 staining solution - by Bioz Stars,
2026-10
99/100 stars
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Incubation:Article Title: Biodegradable Janus sonozyme with continuous reactive oxygen species regulation for treating infected critical-sized bone defects. Article Snippet: .. The cells were covered with primary antibody of the appropriate genus and incubated at room temperature for 60min, washed with 0.1% Phosphate Buffer Saline Tween (PBST), then Article Title: Trans‐Coumaryl acetate mediates GRK5/NF‐κB/Nrf2 signaling axis to ameliorate septic acute kidney injury Article Snippet: Cell surfaces or tissue sections were permeabilized with drops of 0.3% Tritonx‐100 (X100, Sigma‐Aldrich) for 10 min 5% bovine serum albumin (BSA, V900933, Sigma‐Aldrich) was added for closure for 30 min. NF‐κB primary antibody (14‐6731‐81, 1:100, Invitrogen) was added dropwise on the cell surface, and F4/80 primary antibody (MA5‐16363, 1:100, Invitrogen) was added dropwise on the surface of the tissue sections, and incubated overnight at 4°C. .. The next day, the tissue sections were incubated with FITC‐labeled goat anti‐rabbit IgG (F‐2765, 1:100, Invitrogen) for 1 h at 37°C away from light, and then they were exposed to Article Title: Artemether induces JNK1/FOXO1/GPX4-mediated ferroptosis to suppress colorectal cancer. Article Snippet: Background: Colorectal cancer (CRC) remains a leading cause of cancer-related mortality worldwide, necessitating novel therapeutic strategies.. Ferroptosis, an iron‐dependent, lipid-peroxidation-driven form of cell death, has emerged as a potential tumor‐suppressive mechanism.. Artemether, a derivative of Artemisia annua L., exhibits antitumor activity in various cancers, but its role in CRC and its link to ferroptosis remain unclear. Article Title: Heterologous expression of AtLEC1 and AtLEC1-LIKE transcription factors redirects carbon flux toward lipid accumulation in diatom Article Snippet: .. The cell pellet was then treated with 150 μl of Article Title: Heterologous expression of AtLEC1 and AtLEC1-LIKE transcription factors redirects carbon flux toward lipid accumulation in diatom. Article Snippet: .. The cell pellet 175 was then treated with 150 μl of Saline:Article Title: Biodegradable Janus sonozyme with continuous reactive oxygen species regulation for treating infected critical-sized bone defects. Article Snippet: .. The cells were covered with primary antibody of the appropriate genus and incubated at room temperature for 60min, washed with 0.1% Phosphate Buffer Saline Tween (PBST), then Staining:Article Title: Biodegradable Janus sonozyme with continuous reactive oxygen species regulation for treating infected critical-sized bone defects. Article Snippet: .. The cells were covered with primary antibody of the appropriate genus and incubated at room temperature for 60min, washed with 0.1% Phosphate Buffer Saline Tween (PBST), then Article Title: Trans‐Coumaryl acetate mediates GRK5/NF‐κB/Nrf2 signaling axis to ameliorate septic acute kidney injury Article Snippet: Cell surfaces or tissue sections were permeabilized with drops of 0.3% Tritonx‐100 (X100, Sigma‐Aldrich) for 10 min 5% bovine serum albumin (BSA, V900933, Sigma‐Aldrich) was added for closure for 30 min. NF‐κB primary antibody (14‐6731‐81, 1:100, Invitrogen) was added dropwise on the cell surface, and F4/80 primary antibody (MA5‐16363, 1:100, Invitrogen) was added dropwise on the surface of the tissue sections, and incubated overnight at 4°C. .. The next day, the tissue sections were incubated with FITC‐labeled goat anti‐rabbit IgG (F‐2765, 1:100, Invitrogen) for 1 h at 37°C away from light, and then they were exposed to Article Title: Artemether induces JNK1/FOXO1/GPX4-mediated ferroptosis to suppress colorectal cancer. Article Snippet: Background: Colorectal cancer (CRC) remains a leading cause of cancer-related mortality worldwide, necessitating novel therapeutic strategies.. Ferroptosis, an iron‐dependent, lipid-peroxidation-driven form of cell death, has emerged as a potential tumor‐suppressive mechanism.. Artemether, a derivative of Artemisia annua L., exhibits antitumor activity in various cancers, but its role in CRC and its link to ferroptosis remain unclear. Article Title: Human umbilical cord mesenchymal stem cell -derived exosomes alleviated the toxic effects induced by lead on HK-2 cells via regulating the unfolded protein response. Article Snippet: .. Chemicals and reagents utilized in this study are listed below: Lead acetate (Pb(Ac)2, Aladdin, China), High-glucose dulbecco’s modified eagle medium (DMEM, Gibco, USA), Trypsin - EDTA (TE, Gibco, USA), Fetal bovine serum (FBS, Gibco, USA), Penicillin / streptomycin (Gibco, USA), Cell Counting Kit-8 (CCK-8, Abbkine, China), Trizol RNA isolation reagent (Invitrogen, USA), TransScript RT reagent kit (TransGen Biotech, China), SYBR Green qPCR Kit (Accurate Biology, China), Bestar® SybrGreen qPCR Mastermix (Accurate Biology, China), Article Title: Carbon quantum dots exacerbate Parkinson's disease-like pathology through microglia-mediated neuroinflammatory responses with toxicological implications. Article Snippet: The global burden of Parkinson's disease (PD) is projected to double by 2050, with early-onset cases demonstrating accelerated progression and limited therapeutic options.. Recently, carbon quantum dots (CQDs) emerge as promising nanoplatforms for PD theragnostic, but their paradoxical role in exacerbating neuroinflammation and neurodegeneration shows the urgent need for mechanistic safety evaluations to bridge their therapeutic potential and neurotoxic risks.. This study conducted a neuro-safety assessment of CQDs based on MPP-induced mice and cell PD models to stimulate the theragnostic scenarios of CQDs in PD patients in the real-world. Article Title: Therapeutic potential of Platycodin D in allergic asthma through anti-inflammatory and anti-remodeling effects. Article Snippet: Background: Allergic asthma (AA) is a prevalent chronic respiratory disease characterized by airway hyperresponsiveness (AHR) and chronic inflammation, significantly impairing patients’ quality of life.. Purpose: This study investigates the therapeutic effects of Platycodin D (PLD) on AA and its underlying mechanisms via the EGFR/PI3K/Akt signaling pathway.. Methods: In vitro, BEAS-2B cells treated with IL-4 and IL-13 simulated asthma’s inflammatory environment. Article Title: Heterologous expression of AtLEC1 and AtLEC1-LIKE transcription factors redirects carbon flux toward lipid accumulation in diatom Article Snippet: .. The cell pellet was then treated with 150 μl of Article Title: Heterologous expression of AtLEC1 and AtLEC1-LIKE transcription factors redirects carbon flux toward lipid accumulation in diatom. Article Snippet: .. The cell pellet 175 was then treated with 150 μl of Fluorescence:Article Title: Trans‐Coumaryl acetate mediates GRK5/NF‐κB/Nrf2 signaling axis to ameliorate septic acute kidney injury Article Snippet: Cell surfaces or tissue sections were permeabilized with drops of 0.3% Tritonx‐100 (X100, Sigma‐Aldrich) for 10 min 5% bovine serum albumin (BSA, V900933, Sigma‐Aldrich) was added for closure for 30 min. NF‐κB primary antibody (14‐6731‐81, 1:100, Invitrogen) was added dropwise on the cell surface, and F4/80 primary antibody (MA5‐16363, 1:100, Invitrogen) was added dropwise on the surface of the tissue sections, and incubated overnight at 4°C. .. The next day, the tissue sections were incubated with FITC‐labeled goat anti‐rabbit IgG (F‐2765, 1:100, Invitrogen) for 1 h at 37°C away from light, and then they were exposed to Article Title: Therapeutic potential of Platycodin D in allergic asthma through anti-inflammatory and anti-remodeling effects. Article Snippet: Background: Allergic asthma (AA) is a prevalent chronic respiratory disease characterized by airway hyperresponsiveness (AHR) and chronic inflammation, significantly impairing patients’ quality of life.. Purpose: This study investigates the therapeutic effects of Platycodin D (PLD) on AA and its underlying mechanisms via the EGFR/PI3K/Akt signaling pathway.. Methods: In vitro, BEAS-2B cells treated with IL-4 and IL-13 simulated asthma’s inflammatory environment. Microscopy:Article Title: Trans‐Coumaryl acetate mediates GRK5/NF‐κB/Nrf2 signaling axis to ameliorate septic acute kidney injury Article Snippet: Cell surfaces or tissue sections were permeabilized with drops of 0.3% Tritonx‐100 (X100, Sigma‐Aldrich) for 10 min 5% bovine serum albumin (BSA, V900933, Sigma‐Aldrich) was added for closure for 30 min. NF‐κB primary antibody (14‐6731‐81, 1:100, Invitrogen) was added dropwise on the cell surface, and F4/80 primary antibody (MA5‐16363, 1:100, Invitrogen) was added dropwise on the surface of the tissue sections, and incubated overnight at 4°C. .. The next day, the tissue sections were incubated with FITC‐labeled goat anti‐rabbit IgG (F‐2765, 1:100, Invitrogen) for 1 h at 37°C away from light, and then they were exposed to Modification:Article Title: Human umbilical cord mesenchymal stem cell -derived exosomes alleviated the toxic effects induced by lead on HK-2 cells via regulating the unfolded protein response. Article Snippet: .. Chemicals and reagents utilized in this study are listed below: Lead acetate (Pb(Ac)2, Aladdin, China), High-glucose dulbecco’s modified eagle medium (DMEM, Gibco, USA), Trypsin - EDTA (TE, Gibco, USA), Fetal bovine serum (FBS, Gibco, USA), Penicillin / streptomycin (Gibco, USA), Cell Counting Kit-8 (CCK-8, Abbkine, China), Trizol RNA isolation reagent (Invitrogen, USA), TransScript RT reagent kit (TransGen Biotech, China), SYBR Green qPCR Kit (Accurate Biology, China), Bestar® SybrGreen qPCR Mastermix (Accurate Biology, China), CCK-8 Assay:Article Title: Human umbilical cord mesenchymal stem cell -derived exosomes alleviated the toxic effects induced by lead on HK-2 cells via regulating the unfolded protein response. Article Snippet: .. Chemicals and reagents utilized in this study are listed below: Lead acetate (Pb(Ac)2, Aladdin, China), High-glucose dulbecco’s modified eagle medium (DMEM, Gibco, USA), Trypsin - EDTA (TE, Gibco, USA), Fetal bovine serum (FBS, Gibco, USA), Penicillin / streptomycin (Gibco, USA), Cell Counting Kit-8 (CCK-8, Abbkine, China), Trizol RNA isolation reagent (Invitrogen, USA), TransScript RT reagent kit (TransGen Biotech, China), SYBR Green qPCR Kit (Accurate Biology, China), Bestar® SybrGreen qPCR Mastermix (Accurate Biology, China), Isolation:Article Title: Human umbilical cord mesenchymal stem cell -derived exosomes alleviated the toxic effects induced by lead on HK-2 cells via regulating the unfolded protein response. Article Snippet: .. Chemicals and reagents utilized in this study are listed below: Lead acetate (Pb(Ac)2, Aladdin, China), High-glucose dulbecco’s modified eagle medium (DMEM, Gibco, USA), Trypsin - EDTA (TE, Gibco, USA), Fetal bovine serum (FBS, Gibco, USA), Penicillin / streptomycin (Gibco, USA), Cell Counting Kit-8 (CCK-8, Abbkine, China), Trizol RNA isolation reagent (Invitrogen, USA), TransScript RT reagent kit (TransGen Biotech, China), SYBR Green qPCR Kit (Accurate Biology, China), Bestar® SybrGreen qPCR Mastermix (Accurate Biology, China), SYBR Green Assay:Article Title: Human umbilical cord mesenchymal stem cell -derived exosomes alleviated the toxic effects induced by lead on HK-2 cells via regulating the unfolded protein response. Article Snippet: .. Chemicals and reagents utilized in this study are listed below: Lead acetate (Pb(Ac)2, Aladdin, China), High-glucose dulbecco’s modified eagle medium (DMEM, Gibco, USA), Trypsin - EDTA (TE, Gibco, USA), Fetal bovine serum (FBS, Gibco, USA), Penicillin / streptomycin (Gibco, USA), Cell Counting Kit-8 (CCK-8, Abbkine, China), Trizol RNA isolation reagent (Invitrogen, USA), TransScript RT reagent kit (TransGen Biotech, China), SYBR Green qPCR Kit (Accurate Biology, China), Bestar® SybrGreen qPCR Mastermix (Accurate Biology, China), Real-time Polymerase Chain Reaction:Article Title: Human umbilical cord mesenchymal stem cell -derived exosomes alleviated the toxic effects induced by lead on HK-2 cells via regulating the unfolded protein response. Article Snippet: .. Chemicals and reagents utilized in this study are listed below: Lead acetate (Pb(Ac)2, Aladdin, China), High-glucose dulbecco’s modified eagle medium (DMEM, Gibco, USA), Trypsin - EDTA (TE, Gibco, USA), Fetal bovine serum (FBS, Gibco, USA), Penicillin / streptomycin (Gibco, USA), Cell Counting Kit-8 (CCK-8, Abbkine, China), Trizol RNA isolation reagent (Invitrogen, USA), TransScript RT reagent kit (TransGen Biotech, China), SYBR Green qPCR Kit (Accurate Biology, China), Bestar® SybrGreen qPCR Mastermix (Accurate Biology, China), Acid Assay:Article Title: Human umbilical cord mesenchymal stem cell -derived exosomes alleviated the toxic effects induced by lead on HK-2 cells via regulating the unfolded protein response. Article Snippet: .. Chemicals and reagents utilized in this study are listed below: Lead acetate (Pb(Ac)2, Aladdin, China), High-glucose dulbecco’s modified eagle medium (DMEM, Gibco, USA), Trypsin - EDTA (TE, Gibco, USA), Fetal bovine serum (FBS, Gibco, USA), Penicillin / streptomycin (Gibco, USA), Cell Counting Kit-8 (CCK-8, Abbkine, China), Trizol RNA isolation reagent (Invitrogen, USA), TransScript RT reagent kit (TransGen Biotech, China), SYBR Green qPCR Kit (Accurate Biology, China), Bestar® SybrGreen qPCR Mastermix (Accurate Biology, China), Membrane:Article Title: Human umbilical cord mesenchymal stem cell -derived exosomes alleviated the toxic effects induced by lead on HK-2 cells via regulating the unfolded protein response. Article Snippet: .. Chemicals and reagents utilized in this study are listed below: Lead acetate (Pb(Ac)2, Aladdin, China), High-glucose dulbecco’s modified eagle medium (DMEM, Gibco, USA), Trypsin - EDTA (TE, Gibco, USA), Fetal bovine serum (FBS, Gibco, USA), Penicillin / streptomycin (Gibco, USA), Cell Counting Kit-8 (CCK-8, Abbkine, China), Trizol RNA isolation reagent (Invitrogen, USA), TransScript RT reagent kit (TransGen Biotech, China), SYBR Green qPCR Kit (Accurate Biology, China), Bestar® SybrGreen qPCR Mastermix (Accurate Biology, China), Data-independent acquisition:Article Title: Human umbilical cord mesenchymal stem cell -derived exosomes alleviated the toxic effects induced by lead on HK-2 cells via regulating the unfolded protein response. Article Snippet: .. Chemicals and reagents utilized in this study are listed below: Lead acetate (Pb(Ac)2, Aladdin, China), High-glucose dulbecco’s modified eagle medium (DMEM, Gibco, USA), Trypsin - EDTA (TE, Gibco, USA), Fetal bovine serum (FBS, Gibco, USA), Penicillin / streptomycin (Gibco, USA), Cell Counting Kit-8 (CCK-8, Abbkine, China), Trizol RNA isolation reagent (Invitrogen, USA), TransScript RT reagent kit (TransGen Biotech, China), SYBR Green qPCR Kit (Accurate Biology, China), Bestar® SybrGreen qPCR Mastermix (Accurate Biology, China), Glutathione Assay:Article Title: Human umbilical cord mesenchymal stem cell -derived exosomes alleviated the toxic effects induced by lead on HK-2 cells via regulating the unfolded protein response. Article Snippet: .. Chemicals and reagents utilized in this study are listed below: Lead acetate (Pb(Ac)2, Aladdin, China), High-glucose dulbecco’s modified eagle medium (DMEM, Gibco, USA), Trypsin - EDTA (TE, Gibco, USA), Fetal bovine serum (FBS, Gibco, USA), Penicillin / streptomycin (Gibco, USA), Cell Counting Kit-8 (CCK-8, Abbkine, China), Trizol RNA isolation reagent (Invitrogen, USA), TransScript RT reagent kit (TransGen Biotech, China), SYBR Green qPCR Kit (Accurate Biology, China), Bestar® SybrGreen qPCR Mastermix (Accurate Biology, China), ATP Assay:Article Title: Carbon quantum dots exacerbate Parkinson's disease-like pathology through microglia-mediated neuroinflammatory responses with toxicological implications. Article Snippet: The global burden of Parkinson's disease (PD) is projected to double by 2050, with early-onset cases demonstrating accelerated progression and limited therapeutic options.. Recently, carbon quantum dots (CQDs) emerge as promising nanoplatforms for PD theragnostic, but their paradoxical role in exacerbating neuroinflammation and neurodegeneration shows the urgent need for mechanistic safety evaluations to bridge their therapeutic potential and neurotoxic risks.. This study conducted a neuro-safety assessment of CQDs based on MPP-induced mice and cell PD models to stimulate the theragnostic scenarios of CQDs in PD patients in the real-world. |
